METAGENOMIC INVESTIGATION OF PLASMA IN INDIVIDUALS WITH ME/CFS HIGHLIGHTS THE IMPORTANCE OF TECHNICAL CONTROLS TO ELUCIDATE CONTAMINATION AND BATCH EFFECTS.

Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a debilitating disease causing indefinite fatigue.ME/CFS has long been hypothesised to have an infectious cause; however, no specific infectious agent has been identified.We used metagenomics to analyse the RNA from plasma samples from 25 individuals with ME/CFS and compare their microb

Cellular barcoding and other single-cell lineage-tracing strategies form experimental methodologies for analysis of in Worktables vivo cell fate that have been instrumental in several significant recent discoveries.Due to the highly nonlinear nature of proliferation and differentiation, interrogation of the resulting data for evaluation of potentia

Pancreatic cancer (PCa) is a highly lethal and aggressive disease, characterized by high mortality rates.Although necroptosis plays a vital role in tumor progression, cancer metastasis, prognosis of cancer patients, necroptosis-related gene (NRG) sets have rarely been analyzed in PCa.Therefore, definition of novel necroptosis-related prognostic mar